Basic Laboratory Training on Pathogen Analysis in Faecal Sludge and Wastewater

Dates of training: November 1 – 5, 2022
Language: English
Last Date to Apply: October 21, 2022
Venue: Anil Agarwal Environment Training Institute (AAETI), Nimli, Rajasthan

About the training 

Faecal Sludge Management (FSM) is an evolving field with continual development and improvement happening in research methodologies. Faecal Sludge contains large amounts of microorganisms, mainly originating from the faeces. These microorganisms can be pathogenic, and exposure to untreated faecal sludge constitutes a significant health risk to humans, either through direct contact, or through indirect exposure. This course focuses on some of the recent advances in laboratory analysis of detection and enumeration of indicator pathogens present in the faecal sludge and wastewater. This training is part of the Centre for Science and Environment and Bill Melinda Gates Foundation collaboration on FSM in India. In-house experts and lab scientists from CSE’s Environment Monitoring Laboratory (EML) will guide participants through the sessions and help them learn lab analysis using state-of-the-art equipment and methods. The hands-on approach for the programme means that participants get to perform the lab experiments on their own and can enhance their skills while working on some of the best equipment and protocols in their class. The training will be held in CSE’s residential campus in Nimli, near Alwar, Rajasthan.

What you will learn:

• Introduction to faecal sludge management
• Methodologies of safe and proper sample collection and processing
• Laboratory skills on analysis of pathogens in faecal sludgeand wastewater

Methodology:

Highly interactive hands-on training:

• Interactive input presentations
• Field visit and sample collection, storage and transportation for microbial analysis
• Hands-on exercises
• Bacterial (faecal coliform, E. coli) analysis of faecal sludgeand wastewater by MPN method
• Helminth eggs enumeration in faecal sludge by AmBic method
• Bacteriophage (F+) enumeration in wastewater by plaque forming unit (PFU) method
• Enumeration of Clostridium perfringens spores in wastewater by colony count method
• Enumeration of Salmonella spp. in faecal sludge and wastewater by CFU/MPN method
• Individual and group assignments

 

COURSE COORDINATOR	COURSE ORGANISERS
Dr. Vinod Vijayan Senior Research Scientist, vinod_v@cseindia.org Phone: +91 9099115193	Mr. Arvind Singh Senger Senior Research Scientist, arvindsingh.senger@cseindia.org Phone: +91 8879948011 Dr. Ashitha Gopinath Junior Research Scientist, ashitha@cseindia.org Phone: +91 7708319493	Dr. Kalyana Chakravarthy Sama Research Scientist, sama.kalyanachakravarthy@cseindia.org Phone: +91 9849661107 Dr. Megha Tyagi Junior Research Scientist, megha.tyagi@cseindia.org Phone: +91 7708319493